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INTERNATIONAL JOURNAL OF ENGINEERING, SCIENCE AND - Volume 7, Issue 4 (1) , April 2018 (Special Issue FBSA)

Pages: 105-109
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Immobilization of Lipase from Rhizopus arrhizus by Entrapment in Calcium Alginate Beads

Author: Malabika Chakraborty

Category: Engineering, Science and Mathematics


An extracellular lipase producing mutant stain of Rhizopus arrhizus isolated and purified was immobilised in Ca-alginate beads by Gel entrapment process. The alginate beads were prepared as sodium alginate in crude enzyme extract and CaCl2 to increase its reusability and stability. In the presence of Ca+2 cations, the enzyme-alginate suspension was crosslinked to form beads at room temperature. The effects of reaction parameters such as Na-alginate and CaCl2 concentrations were optimized and the corresponding bead sizes were measured for the alginate maximum production of lipase by immobilized enzyme. The optimum concentration of Na-alginate and CaCl2 were found to be 6% (w/v) and 0.4M respectively. The maximum production of immobilized lipase was observed with bead size 1.5mm. After immobilization, 60-% lipase activity was retained. The entrapped lipase was stable over a wide range of temperature (300c – 600c) and pH (5-9.5). The Ca-alginate entrapped fungal lipase can be used in the industry.

Keywords: Immobilization Lipase Rhizopus arrhizus Entrapment Calcium