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INTERNATIONAL JOURNAL OF ENGINEERING, SCIENCE AND - Volume 7, Issue 4 (1) , April 2018 (Special Issue FBSA)

Pages: 84-95
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Characterize heavy metal tolerant rhizospheric sewage bacteria isolated from Tolly nullah (with special emphasis on strain Microbacterium radiodurans K12016)

Author: Chitrita Chatterjee? Rabindranath Bhattacharyya?? Shampa Sarkar (Biswas)???

Category: Engineering, Science and Mathematics


Because of rapid industrialization and urbanization, heavy metals are deposited in the environment in frightening quantity. Literature survey manifested, that the heavy metal contaminated soil born microbes can be a potent mode of environmental heavy metal degradation. In the present study, to reveal microbial heavy metal tolerant activity rhizosperic microbes were isolated from sewage sludge of circular canal (Tolly Nullah) and characterize. Microbial heavy metal resistance pattern was checked against four heavy metal salts (Cr2O3, CrO3, CdCl2 and CoCl2) individually and in consortium. Plant growth promoting rhizobacterial (PGPR) traits of isolated microbes were evaluated. One interesting isolated strain (Microbacterium radiodurans strain K12016), identified by 16s rDNA, shown total tolerance against 100 mM Cr2O3 (Cr3+) and also mentionable Cobalt and hexavalent Chromium (Cr6+) tolerance, but didn’t produce a significant amount of plant growth promoting IAA whereas the most of the rest isolates produced adequate amount of IAA. 16s rDNA of that particular isolate was analyzed and phylogenic tree was constructed using the neighbor-joining method to ensure its taxonomic position. Furthermore experimentation and gene study should require to determining heavy metal resistance mechanism of Microbacterium radiodurans K12016.

Keywords: Heavy Metal tolerance; Biochemical analysis; Antibiotic resistance; Indole Acetic Acid (IAA); 16s rDNA.