In present scenario, the use of Supercritical fluids are increased enormously due to their desired thermophysical properties such as higher density with lower viscosity. The need of Supercritical Carbon dioxide (SCCO₂) in cryocoolers to be used in Natural Circulation cryogenic refrigeration system is still need to be studied to determine the heat transfer rate for designing better cryocooler. The heat transfer rate depends on thermophysical properties of SCCO₂. Hence, it is necessary to predict the temperature dependent thermophysical properties of SCCO₂ such as Thermal conductivity, viscosity, density and specific heat. In this context, study is carried out with a wide temperature ranges from 305K to 350K and pressure ranges from 75 bar to 100 bar with a raise in 5K temperature and 5 bar pressure. The heat transfer rate is predicted by analyzing the natural circulation loop modeled and analyzed in commercial software above to critical operating pressure and temperature of SCCO₂. Boundary conditions are imposed on the model to compatible with the real time application. From the investigation it is concluded that SCCO₂ is having better properties than carbon dioxide and the heat transfer rate is also enhanced. Hence, the use of SCCO₂in cryocooler of cryogenic refrigeration system is better compared to CO₂.