INTERNATIONAL JOURNAL OF ENGINEERING, SCIENCE AND - Volume 4, Issue 1 , March 2015

Pages: 82-95

Date of Publication: 01-Mar-2015

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A REVIEW ON PROTOCOLS FOR IN VITRO REGENERATION OF PLANTS

Author: S.B SADAU* Sani, I*

Category: Engineering, Science and Mathematics

Abstract:

In the present review, an attempt has been made to set up protocols for in vitro regeneration of plant from young leaves, seedlings as explant source. For in vitro callus inductions auxins such as IAA, NAA and 2, 4-D in combination with cytokines like BAP and KN areused. Awell-developed greenish plant is obtained on MS medium when supplemented with BAP and NAA.The maximum number of shoot bud regeneration (14.0 shoots /explant) is observed on MS medium containing 5.0mg/l BAP, ofthe two cytokines used, BAP is found to be best for shoot bud regeneration.

Keywords: in vitro culture, plant regeneration, callus induction, multiple shoots, indirect organogenesis