INTERNATIONAL JOURNAL OF ENGINEERING, SCIENCE AND - Volume 3, Issue 4 , December 2014

Pages: 115-122

Date of Publication: 01-Dec-2014

Print Article   Download XML  Download PDF

KINETICS OF OXIDATION OF THIAMINE BY PHOSPHATE RADICAL ANIONS PROTECTION BY CAFFECIC ACID

Author: Biyyala Sreedhar Rao* M. Anand Rao*

Category: Engineering, Science and Mathematics

Abstract:

The oxidation of thymidine by PO4•− has been followed by measuring the absorbance of thymiine at 264 nm to 310 nm spectrophotometrically. The rates and the quantum yields (φ) of oxidation of thymine by phosphate radical anion have been determined in the presence of different concentrations of caffeic acid. Increase in [caffeic acid] is found to decrease the rate of oxidation of thymine suggesting that caffeic acid acts as an efficient scavenger of PO4•− and protects thymine from it. Phosphate radical anion competes for thymine as well as for caffeic acid. The quantum yields of photooxidation of thymine have been calculated from the rates of oxidation of thymidine and the light intensity absorbed by Peroxodiphosphate (PDP) at 264 nm, the wavelength at which PDP is activated to sulphate radical anion.

Keywords: Kinetics, thiamine hydrochloride (vit – B1), PDP and caffeic acid.